Importantly, colocalization results cannot indicate that two proteins/molecules are bound or interacting, only that they are both localized to within a certain volume, and is mostly dependent upon your …

In fluorescence microscopy, colocalization refers to observation of the spatial overlap between two (or more) different fluorescent labels, each having a separate emission wavelength, to see if the different …

Here we provide a guide to analyzing colocalization in cell biology studies, emphasizing practical application of quantitative tools that are now widely available in commercial and free image analysis …

Colocalization studies rely on image preprocessing and further analysis; however, they are limited by optical resolution. Once those limitations are taken into account, characterization might be …

Colocalization is when two or more different labels (e.g., eGFP and mCherry) spatially overlap in your image (also called co-occurrence). Another component of colocalization is that the fluorescent labels …

Jul 24, 2025 · Colocalization in biology refers to the observation that two or more different molecules are present in the exact same location within a cell at the same time. Like two individuals consistently …

Dunn, K. W., Kamocka, M. M., and McDonald, J. H., A Practical Guide To Evaluating Colocalization In Biological Microscopy. Am J Physiol Cell Physiol. 2011 Apr; 300(4): C723–C742 Bolte, S., and …